PROTONATED TRIPLEX DNA IN ESCHERICHIA-COLI-CELLS AS DETECTED BY CHEMICAL PROBING

被引：41
|
作者：
KARLOVSKY, P
PECINKA, P
VOJTISKOVA, M
MAKATUROVA, E
PALECEK, E
机构：
[1] CZECHOSLOVAK ACAD SCI, INST BIOPHYS, KRALOVOPOLSKA 135, CS-61265 BRNO, CZECHOSLOVAKIA
[2] GESELL STRAHLEN & UMWELTFORSCH MBH, MOLEK GENET ABT, W-3400 GOTTINGEN, GERMANY
来源：
FEBS LETTERS | 1990年 / 274卷 / 1-2期
关键词：
E; coli; H-DNA conformer; Homopurine-homopyrimidine sequence; Intracellular pH; Intracellular plasmid; Osmium tetroxide; Triplex DNA;
D O I：
10.1016/0014-5793(90)81324-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from that observed in vitro. © 1990.
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页码：39 / 42
页数：4
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