PROTONATED TRIPLEX DNA IN ESCHERICHIA-COLI-CELLS AS DETECTED BY CHEMICAL PROBING

被引：41
|
作者：
KARLOVSKY, P
PECINKA, P
VOJTISKOVA, M
MAKATUROVA, E
PALECEK, E
机构：
[1] CZECHOSLOVAK ACAD SCI, INST BIOPHYS, KRALOVOPOLSKA 135, CS-61265 BRNO, CZECHOSLOVAKIA
[2] GESELL STRAHLEN & UMWELTFORSCH MBH, MOLEK GENET ABT, W-3400 GOTTINGEN, GERMANY
来源：
FEBS LETTERS | 1990年 / 274卷 / 1-2期
关键词：
E; coli; H-DNA conformer; Homopurine-homopyrimidine sequence; Intracellular pH; Intracellular plasmid; Osmium tetroxide; Triplex DNA;
D O I：
10.1016/0014-5793(90)81324-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from that observed in vitro. © 1990.
引用
收藏
页码：39 / 42
页数：4
相关论文
共 50 条
  • [1] INTRAMOLECULAR DG.DG.DC TRIPLEX DETECTED IN ESCHERICHIA-COLI-CELLS
    KOHWI, Y
    MALKHOSYAN, SR
    KOHWISHIGEMATSU, T
    JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (04) : 817 - 822
  • [2] BRANCHED ESCHERICHIA-COLI-CELLS
    AKERLUND, T
    NORDSTROM, K
    BERNANDER, R
    MOLECULAR MICROBIOLOGY, 1993, 10 (04) : 849 - 858
  • [3] EXPRESSION OF ORTHOPOXVIRUS DNA-SEQUENCES IN ESCHERICHIA-COLI-CELLS
    SHCHELKUNOV, SN
    RYAZANKINA, OI
    KRYKBAEV, RA
    BELAVIN, PA
    MOLECULAR BIOLOGY, 1992, 26 (04) : 528 - 532
  • [4] REGULATION OF DNA LIGATION BY THE RECB PROTEIN IN ESCHERICHIA-COLI-CELLS
    SYVAOJA, J
    PYHTILA, MJ
    FEBS LETTERS, 1985, 183 (02): : 383 - 386
  • [5] KINETOPLAST DNA SEGMENTS FUNCTION AS PROMOTERS IN ESCHERICHIA-COLI-CELLS
    KUZMIN, EV
    TARASSOFF, IA
    ZAITSEVA, GN
    MOLECULAR AND GENERAL GENETICS, 1985, 198 (02): : 323 - 328
  • [6] COMPETENCE IN ESCHERICHIA-COLI-CELLS .1. RECOVERY OF TRANSFECTIVITY IN INCOMPETENT ESCHERICHIA-COLI-CELLS
    RUDCHENK.ON
    LIKHACHE.NA
    TIMAKOVA, NV
    ILYASHEN.BN
    GENETIKA, 1974, 10 (02): : 139 - 144
  • [7] CLONING OF BORDETELLA-PERTUSSIS DNA IN THE SYSTEM OF ESCHERICHIA-COLI-CELLS
    SEMINA, IE
    TARASOV, AP
    MILEIKOVSKAYA, MM
    KAZENNOVA, EV
    TSVETKOVA, NV
    ZHURNAL MIKROBIOLOGII EPIDEMIOLOGII I IMMUNOBIOLOGII, 1983, (08): : 17 - &
  • [8] DNA-REPAIR PROCESSES IN ESCHERICHIA-COLI-CELLS INDUCED BY DAMAGE
    SLEZARIKOVA, V
    BIOLOGIA, 1986, 41 (08) : 825 - 829
  • [9] INTERACTION OF LIPOSOMES WITH ESCHERICHIA-COLI-CELLS - THE EFFECT EXOGENOUS DNA UPTAKE
    SABELNIKOV, AG
    VASILENKO, IA
    MISHINA, IM
    SHVETS, VI
    BIOLOGICHESKIE MEMBRANY, 1988, 5 (04): : 408 - 419
  • [10] ELECTROSTIMULATED TRANSFORMATION OF ESCHERICHIA-COLI-CELLS
    TSYMBALYUK, ES
    CHERNOMORDIK, LV
    BROUDE, NE
    CHIZMADZHEV, YA
    BIOLOGICHESKIE MEMBRANY, 1988, 5 (03): : 240 - 245
12345