EFFECT OF SERUM ON DIFFERENTIATION OF PORCINE ADIPOSE STROMAL-VASCULAR CELLS IN PRIMARY CULTURE

被引：64

作者：

SURYAWAN, A ^{[1
]}

HU, CY ^{[1
]}

机构：

[1] OREGON STATE UNIV,DEPT ANIM SCI,WITHYCOMBE HALL 308,CORVALLIS,OR 97331

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1993年 / 105卷 / 03期

关键词：

D O I：

10.1016/0300-9629(93)90424-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Serum-containing media without additional hormones failed to support the differentiation of porcine SV cells while hormone-supplemented serum-free medium (ITTC) stimulated differentiation as indicated by higher GPDH specific activity. 2. All sera tested stimulated proliferation and inhibited porcine SV cell differentiation to some degree; fetal calf and rat sera were more inhibitory than calf and pig sera. 3. Morphologicaly, in serum-free medium porcine SV cells developed into individual adipocytes while in all serum-containing media they formed fat cell clusters. 4. Insulin and hydrocortisone in serum-free medium were important factors in SV cell differentiation while transferrin, T3, and fibroblast growth factor (FGF) were less important.

引用

页码：485 / 492

页数：8

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