Increasing Uniformity of Biosurfactant Production in Starmerella bombicola via the Expression of Chimeric Cytochrome P450s

被引:13
|
作者
Geys, Robin [1 ]
De Graeve, Marilyn [1 ]
Lodens, Sofie [1 ]
Van Malderen, Jeroen [1 ]
Lemmens, Christophe [1 ]
De Smet, Margaux [1 ]
Mincke, Stein [2 ]
Van Bogaert, Inge N. A. [1 ]
Stevens, Christian [2 ]
De Maeseneire, Sofie L. [1 ]
Roelants, Sophie L. K. W. [1 ]
Soetaert, Wim K. G. [1 ]
机构
[1] Univ Ghent, Fac Biosci Engn, Ctr Ind Biotechnol & Biocatalysis InBiobe, Dept Biotechnol, Coupure Links 653, B-9000 Ghent, Belgium
[2] Univ Ghent, Fac Biosci Engn, Dept Green Chem & Technol, Sustainable Organ Chem & Technol, Coupure Links 653, B-9000 Ghent, Belgium
来源
COLLOIDS AND INTERFACES | 2018年 / 2卷 / 04期
基金
欧盟地平线“2020”;
关键词
sophorolipid; biosurfactant; Starmerella bombicola; cytochrome P450; chimeragenesis; enzyme engineering; process engineering; fermentation; purification; NMR;
D O I
10.3390/colloids2040042
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Sophorolipids are one of the best known microbial biosurfactants and are produced by several yeast species. The best studied producer is Starmerella bombicola, a non-pathogenic yeast associated in nature with bumblebees. Sophorolipids are built up of the rare disaccharide sophorose, which is attached to a fatty acid through a glyosidic bound. Sophorolipids produced by S. bombicola mainly contain oleic acid as the incorporated hydrophobic group. Other chain lengths can, to a certain content, be incorporated by feeding the yeast with substrates of alternative chain lengths. However, the efficiency for such substrates is low as compared to the preferred C18 chain length and defined by the substrate specificity of the first enzymatic step in sophorolipid biosynthesis, i.e., the cytochrome P450 enzyme CYP52M1. To increase product uniformity and diversity at the same time, a new strain of S. bombicola was developed that produces sophorolipids with a palmitic acid acyl chain. This was achieved by heterologous expression of the cytochrome P450 cyp1 gene of Ustilago maydis and feeding with palmitic acid. Optimization of the production was done by protein and process engineering.
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页数:13
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