A RAPID METHOD FOR THE DETERMINATION OF METHOPRENE IN TOBACCO BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：8
|
作者：
YANG, SS
机构：
[1] Research Center, Philip Morris USA, Richmond, 23261-6583, VA
来源：
CHROMATOGRAPHIA | 1992年 / 33卷 / 7-8期
关键词：
COLUMN LIQUID CHROMATOGRAPHY; METHOPRENE; TOBACCO;
D O I：
10.1007/BF02275907
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid sample preparation procedure combined with a short reversed-phase HPLC separation for the quantitation of methoprene residue in tobacco samples is described. A ground tobacco sample of 0.5 g is mixed with 3 mL of 2-propanol. The mixture is extracted for twelve minutes with the aid of sonication at an elevated temperature (45-55-degrees-C) and then filtered through a 0.45-mu-m disposable filter prior to injection on HPLC. No sample cleanup or solvent evaporation step is required. Chromatographic analysis is performed on a C-18 column and the analysis time is 12.5 minutes. The detection limit for methoprene in tobacco samples is one part per million (mu-g/g).
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页码：309 / 312
页数：4
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