STUDY OF SPADNS AS MOBILE-PHASE COMPLEXING AGENT FOR METAL-ION SEPARATION IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The possibility of employing SPADNS as a mobile-phase complexing agent in RP-HPLC determination of metal ions has been investigated. Separation of Fe(II), Cu(II), and SPADNS has been performed using a 50:50 ( v v) methanol-water mixture 0.02 M in TBA and 10-5 M in SPADNS as mobile phase. Sensitivity was very poor with this mobile phase. When the SPADNS concentration in the solution was increased to 10-3 M, sensitivity improved and detection limits of 2.1 and 1.7 ppm for Fe(III) and Cu(II), respectively, were obtained. However, no selectivity was obtained under these conditions. © 1991.