MOLECULAR MECHANISM OF PROTEIN-KINASE-C MODULATION OF SODIUM-CHANNEL ALPHA-SUBUNITS EXPRESSED IN XENOPUS OOCYTES

被引：34
|
作者：
SCHREIBMAYER, W [1 ]
DASCAL, N [1 ]
LOTAN, I [1 ]
WALLNER, M [1 ]
WEIGL, L [1 ]
机构：
[1] TEL AVIV UNIV,SACKLER SCH MED,DEPT PHYSIOL & PHARMACOL,IL-69978 TEL AVIV,ISRAEL
来源：
FEBS LETTERS | 1991年 / 291卷 / 02期
关键词：
SODIUM CHANNEL MODULATION; PROTEIN KINASE-C (PKC); PHORBOL ESTER; XENOPUS-LAEVIS; PATCH CLAMP; RNA EXPRESSION;
D O I：
10.1016/0014-5793(91)81316-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mechanism of modulation of sodium channel alpha-subunits (Type IIA) by a protein kinase C (PKC) activator was studied on single channel level. It was found that: (i) time constants for channel activation were prolonged; (ii) inactivation remained virtually unchanged; (iii) peak sodium inward current was reduced as evidenced by calculation of average sodium currents; and (iv) time constants for current activation and decay were prolonged. (i), (iii) and (iv) were voltage dependent, being most prominent at threshold potentials. The data show that a voltage dependent action on the activation gate can account for the observed reduction of peak inward sodium current and prolongation of current decay in macroscopic experiments.
引用
收藏
页码：341 / 344
页数：4
相关论文
共 50 条
  • [31] VOLTAGE-DEPENDENT REGULATION OF MODAL GATING IN THE RAT SKM1 SODIUM-CHANNEL EXPRESSED IN XENOPUS OOCYTES
    JI, S
    SUN, WJ
    GEORGE, AL
    HORN, R
    BARCHI, RL
    JOURNAL OF GENERAL PHYSIOLOGY, 1994, 104 (04): : 625 - 643
  • [32] Activation of protein kinase A modulates trafficking of the human cardiac sodium channel in Xenopus oocytes
    Zhou, JS
    Yi, JX
    Hu, NN
    George, AL
    Murray, KT
    CIRCULATION RESEARCH, 2000, 87 (01) : 33 - 38
  • [33] INHIBITION OF SODIUM PUMPS OF XENOPUS OOCYTES BY STIMULATION OF PROTEIN KINASE-C
    VASILETS, L
    SCHMALZING, G
    SCHWARZ, W
    BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1989, 370 (09): : 967 - 968
  • [34] Activation of protein kinase C induces internalization of the GABAC receptors expressed in Xenopus oocytes
    Kusama, T
    Hatama, K
    Saito, K
    Kizawa, Y
    Murakami, H
    JAPANESE JOURNAL OF PHYSIOLOGY, 2000, 50 (04): : 429 - 435
  • [35] THE ADULT-RAT BRAIN BETA(1) SUBUNIT MODIFIES ACTIVATION AND INACTIVATION GATING OF MULTIPLE SODIUM-CHANNEL ALPHA-SUBUNITS
    PATTON, DE
    ISOM, LL
    CATTERALL, WA
    GOLDIN, AL
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (26) : 17649 - 17655
  • [36] INHIBITION OF PROTEIN KINASE C (PKC) RESPONSE OF VOLTAGE GATED CALCIUM (CAv)2.2 CHANNELS EXPRESSED IN XENOPUS OOCYTES BY CAvβ SUBUNITS
    Rajagopal, S.
    Fields, B. L.
    Burton, B. K.
    On, C.
    Reeder, A. A.
    Kamatchi, G. L.
    NEUROSCIENCE, 2014, 280 : 1 - 9
  • [37] MODULATION OF A SHAKER POTASSIUM A-CHANNEL BY PROTEIN-KINASE-C ACTIVATION
    MORAN, O
    DASCAL, N
    LOTAN, I
    FEBS LETTERS, 1991, 279 (02) : 256 - 260
  • [38] PROTEIN-KINASE-C INITIALLY INHIBITS THE INDUCTION OF MEIOTIC CELL-DIVISION IN XENOPUS OOCYTES
    STITH, BJ
    GOALSTONE, ML
    KIRKWOOD, AJ
    CELLULAR SIGNALLING, 1992, 4 (04) : 393 - 403
  • [39] VOLTAGE-DEPENDENT REGULATION OF MODAL GATING IN THE RAT SKM1 SODIUM-CHANNEL EXPRESSED IN XENOPUS-OOCYTES
    JI, S
    SUN, W
    GEORGE, AL
    HORN, R
    BARCHI, RL
    BIOPHYSICAL JOURNAL, 1994, 66 (02) : A243 - A243
  • [40] DESENSITIZATION OF ENDOGENOUS ANGIOTENSIN-II RECEPTORS IN XENOPUS OOCYTES - A ROLE OF PROTEIN-KINASE-C
    SAKUTA, H
    SEKIGUCHI, M
    OKAMOTO, K
    SAKAI, Y
    EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1991, 208 (01): : 41 - 47
12345