MOLECULAR MECHANISM OF PROTEIN-KINASE-C MODULATION OF SODIUM-CHANNEL ALPHA-SUBUNITS EXPRESSED IN XENOPUS OOCYTES

被引：34
|
作者：
SCHREIBMAYER, W [1 ]
DASCAL, N [1 ]
LOTAN, I [1 ]
WALLNER, M [1 ]
WEIGL, L [1 ]
机构：
[1] TEL AVIV UNIV,SACKLER SCH MED,DEPT PHYSIOL & PHARMACOL,IL-69978 TEL AVIV,ISRAEL
来源：
FEBS LETTERS | 1991年 / 291卷 / 02期
关键词：
SODIUM CHANNEL MODULATION; PROTEIN KINASE-C (PKC); PHORBOL ESTER; XENOPUS-LAEVIS; PATCH CLAMP; RNA EXPRESSION;
D O I：
10.1016/0014-5793(91)81316-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mechanism of modulation of sodium channel alpha-subunits (Type IIA) by a protein kinase C (PKC) activator was studied on single channel level. It was found that: (i) time constants for channel activation were prolonged; (ii) inactivation remained virtually unchanged; (iii) peak sodium inward current was reduced as evidenced by calculation of average sodium currents; and (iv) time constants for current activation and decay were prolonged. (i), (iii) and (iv) were voltage dependent, being most prominent at threshold potentials. The data show that a voltage dependent action on the activation gate can account for the observed reduction of peak inward sodium current and prolongation of current decay in macroscopic experiments.
引用
收藏
页码：341 / 344
页数：4
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