DETECTION OF SMALL GTP-BINDING PROTEINS IN THE OUTER ENVELOPE MEMBRANE OF PEA-CHLOROPLASTS

被引：17
|
作者：
SASAKI, Y
SEKIGUCHI, K
NAGANO, Y
MATSUNO, R
机构：
[1] Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto
来源：
FEBS LETTERS | 1991年 / 293卷 / 1-2期
关键词：
CHLOROPLAST OUTER MEMBRANE; SMALL GTP-BINDING PROTEIN; PEA;
D O I：
10.1016/0014-5793(91)81166-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We found small GTP-binding proteins in the outer envelope membrane of pea chloroplasts. The proteins in this membrane were separated by SDS-PAGE, transferred to a nitrocellulose filter, and incubated with [alpha-P-32]GTP. Three GTP-binding proteins with the molecular weight of 24 000 were found. Binding was prevented by 10(-8)-10(-7) M GTP or by 10(-7) M guanosine 5'-[gamma-thio]triphosphate or GDP; binding was unaffected by 10(-8)-10(-6) M ATP. Thermolysin treatment of intact chloroplasts resulted in the loss of GTP-binding activity, suggesting that these proteins were in the cytosolic side of the outer envelope membrane.
引用
收藏
页码：124 / 126
页数：3
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