KINETIC-PROPERTIES OF YEAST LYSINE PERMEASES CODED BY GENES ON MULTI-COPY VECTORS

被引：0
|
作者：
SYCHROVA, H [1 ]
MATEJCKOVA, A [1 ]
KOTYK, A [1 ]
机构：
[1] CZECHOSLOVAK ACAD SCI, INST PHYSIOL, DEPT MEMBRANE TRANSPORT, VIDENSKA 1083, CS-14220 PRAGUE 4, CZECHOSLOVAKIA
来源：
FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 01期
关键词：
LYSINE PERMEASE; SACCHAROMYCES-CEREVISIAE; CANDIDA-ALBICANS;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Amplification of the LYP1 transport system in the plasma membrane of Saccharomyces cerevisiae did not change the substrate specificity, the affinity and the pH optimum of the transport system for lysine, but significantly increased the uptake velocity and accumulation ratio. The dependence of active lysine uptake and accumulation on pH is probably given by the properties of the permease itself rather than by the value of available protonmotive force.
引用
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页码：57 / 61
页数：5
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