RAPID 16S RIBOSOMAL DNA SEQUENCING FROM A SINGLE COLONY WITHOUT DNA EXTRACTION OR PURIFICATION

被引：0

作者：

FROTHINGHAM, R

ALLEN, RL

WILSON, KH

机构：

[1] DUKE UNIV,MED CTR,DEPT MED,DIV INFECT DIS,DURHAM,NC 27710

[2] VET ADM MED CTR,MED SERV,DURHAM,NC 27705

[3] VET ADM MED CTR,RES SERV,DURHAM,NC 27705

来源：

BIOTECHNIQUES | 1991年 / 11卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribosomal RNA sequences are useful for establishing phylogenetic relationships, for oligonucleotide probes and for characterization of uncultured organisms. We describe rapid ribosomal DNA sequencing using PCR with transcript sequencing. Nucleic acid specificity at three steps (amplification, transcription and sequencing) eliminated the need for nucleic acid extraction or purification. Sequence was obtained from a crude lysate from a single colony of bacteria. The basic sequencing method should be adaptable to provide rapid sequence information in a wide variety of applications.

引用

页码：40 / &

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