REGULATION BY CYTOKINES OF EOSINOPHILOPOIESIS AND IMMUNOGLOBULIN-E PRODUCTION IN MICE

被引:0
|
作者
TAKENAKA, T [1 ]
KURIBAYASHI, K [1 ]
NAKAMINE, H [1 ]
TSUJIMOTO, M [1 ]
FUKUHARA, Y [1 ]
MAEDA, J [1 ]
MIHARA, M [1 ]
UCHIYAMA, Y [1 ]
OHSUGI, Y [1 ]
机构
[1] CHUGAI PHARMACEUT CO LTD, SHIZUOKA, JAPAN
关键词
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Certain clinical disorders are associated with eosinophilia and hyperimmunoglobulinaemia of the immunoglobulin (Ig) E class, but others give rise to eosinophilia without hyperimmunoglobulinaemia. To investigate why there are two such patterns, we immunized BALB/c mice with ovalbumin plus tetanus toxid with aluminium hydroxide gel (alum) as adjuvant, which caused eosinophilopoiesis and hyperimmunoglobulinaemia, and also immunized mice with the same antigens and complete Freund's adjuvant (CFA), which caused only eosinophilopoiesis. The difference in the adjuvant brought about the two different patterns. Monoclonal antibodies to four cytokines were used to find that eosinophilopoiesis was mediated by interleukin-5 (IL-5), whichever the adjuvant, and that IgE production in mice treated with alum was regulated by both IL-4 and IL-5. The lack of increase in IgE production was not due to suppression by interferon-gamma (IFN-gamma) or by CD8+ T cells. By the polymerase chain reaction, both IL-4 and IL-5 mRNA were detected in cells from mice given alum, but only IL-5 mRNA was found in cells from mice given CFA. Thus, alum used as the adjuvant resulted in both eosinophilopoiesis and IgE production, but CFA resulted in eosinophilopoiesis alone. The dissociation between eosinophilopoiesis and IgE production shown in the mice given CFA could be due to the dissociation between the expression of mRNA of the cytokines IL-4 and IL-5 in vivo.
引用
收藏
页码:541 / 546
页数:6
相关论文
共 50 条
  • [21] HETEROGENEITY OF IMMUNOGLOBULIN-E
    BERGMANN, KC
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1977, 6 (11) : 1172 - 1172
  • [22] IMMUNOGLOBULIN-E AND REAGINIC HYPERSENSITIVITY
    ISHIZAKA, K
    JOHNS HOPKINS MEDICAL JOURNAL, 1974, 135 (02): : 67 - 90
  • [23] Stromal cell regulation of eosinophilopoiesis is modulated by inflammatory cytokines
    Landreth, K
    Piktel, D
    Gibson, L
    Weissman, D
    Welch, J
    Hogan, M
    EXPERIMENTAL HEMATOLOGY, 2003, 31 (07) : 219 - 219
  • [24] IMMUNOGLOBULIN-E (REAGIN) AND ALLERGY
    STANWORTH, DR
    NATURE, 1971, 233 (5318) : 310 - +
  • [25] TRANSFUSED IMMUNOGLOBULIN-E IN NEWBORNS
    HAMBURGER, RN
    ORGEL, HA
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1982, 70 (02) : 143 - 145
  • [26] IMMUNOGLOBULIN-E IN NIGERIAN ONCHOCERCIASIS
    SOMORIN, AO
    HEINER, DC
    AJUGWO, RE
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1977, 26 (05): : 872 - 876
  • [27] DERMOGRAPHIA MEDIATED BY IMMUNOGLOBULIN-E
    NEWCOMB, RW
    NELSON, H
    AMERICAN JOURNAL OF MEDICINE, 1973, 54 (02): : 174 - 180
  • [28] GENETIC STUDY OF IMMUNOGLOBULIN-E
    GERRARD, JW
    RAO, DC
    MORTON, NE
    AMERICAN JOURNAL OF HUMAN GENETICS, 1978, 30 (01) : 46 - 58
  • [29] SERUM IMMUNOGLOBULIN-E IN PSORIASIS
    DELUCA, M
    SATRIANO, RA
    MAZZA, M
    TASSI, GC
    PISANI, M
    ANNALES DE DERMATOLOGIE ET DE VENEREOLOGIE, 1978, 105 (6-7): : 655 - 656
  • [30] IMMUNOGLOBULIN-E A NEW CLASS OF HUMAN IMMUNOGLOBULIN
    BENNICH, HH
    ISHIZAKA, K
    JOHANSSON, SG
    ROWE, DS
    STANWORTH, DR
    TERRY, WD
    INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1969, 35 (03): : 310 - +