ASSAY OF PANCREATIC LIPASE WITH THE SURFACE-ACOUSTIC-WAVE SENSOR SYSTEM

被引：21
|
作者：
GE, K [1 ]
LIU, DH [1 ]
CHEN, K [1 ]
NIE, LH [1 ]
YAO, SZ [1 ]
机构：
[1] HUNAN UNIV,DEPT CHEM & CHEM ENGN,CHANGSHA 410082,PEOPLES R CHINA
来源：
ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 02期
关键词：
D O I：
10.1006/abio.1995.1215
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new type of surface acoustic wave sensor system for assaying the activity of pancreatic lipase has been proposed. The assay of this enzyme is based on the change in conductance of the solution caused by the release of a fatty acid, using triolein as a substrate. A linear relationship between frequency response and enzyme concentration is obtained. Kinetic parameters of pancreatic lipase, i.e., the Michaelis constant for triolein and the corresponding maximum initial rate, are estimated to be 5.46 mM and 7551.6 Hz/min, respectively. The effects of temperature and pH value are also investigated. (C) 1995 Academic Press, Inc.
引用
收藏
页码：207 / 211
页数：5
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