CONTINUOUS-FLOW SYSTEM FOR LARGE-SCALE UV IRRADIATION OF BACTERIAL-CELLS

被引：0
|
作者：
JOSHI, JG
SWENSON, PA
机构：
[1] UNIV TENNESSEE, DEPT BIOCHEM, KNOXVILLE, TN 37916 USA
[2] OAK RIDGE NATL LAB, DIV BIOL, OAK RIDGE, TN 37830 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1977年 / 33卷 / 04期
关键词：
D O I：
10.1128/AEM.33.4.915-917.1977
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A quartz flow-cell system for UV irradiation of large volumes of Escherichia coli cultures is described. With this system kilogram quantities of irradiated cells can be obtained for biochemical studies. Changes in respiration and in specific activities of superoxide dismutase and catalase, after UV treatment that reduced viability of culture samples to 0.2%, agreed well with those for cultures irradiated (52 J/m2) by a conventional small-scale method to produce the same viability reduction.
引用
收藏
页码：915 / 917
页数：3
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