POLYMORPHIC MICROSATELLITE LOCI OF THE RAT (RATTUS-NORVEGICUS)

被引：39
|
作者：
KUNIEDA, T
KOBAYASHI, E
TACHIBANA, M
IKADAI, H
IMAMICHI, T
机构：
[1] Imamichi Institute for Animal Reproduction, Niihari-gun, Ibaraki, 300-01, Fukyaa 1103, Dejima-mura
来源：
MAMMALIAN GENOME | 1992年 / 3卷 / 10期
关键词：
D O I：
10.1007/BF00350622
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The EMBL and GenBank DNA databases were searched for microsatellite sequences of the rat containing dinucleotide repeats of (CA)n and (GA)n. Among those obtained, 23 sequences were analyzed by polymerase chain reaction to examine the size variation of the amplified fragment in inbred rat strains. All of the 23 microsatellite sequences varied in size among the strains tested. The 23 microsatellite loci in a pair of substrains separated from the same progenitor strain were then analyzed. Fragments identical in size were observed in all loci of the two substrains, indicating the stability of the microsatellite over a large number of generations. The microsatellite loci, therefore, should be useful markers for linkage analyses in the rat.
引用
收藏
页码：564 / 567
页数：4
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