VALIDATION OF A RADIOASSAY FOR THE DETERMINATION OF SERUM FOLATE AND COBALAMIN CONCENTRATIONS IN DOGS

被引:14
|
作者
BATT, RM [1 ]
MCLEAN, L [1 ]
RUTGERS, HC [1 ]
HALL, EJ [1 ]
机构
[1] UNIV LIVERPOOL,DEPT VET PATHOL,LIVERPOOL L69 3BX,ENGLAND
关键词
D O I
10.1111/j.1748-5827.1991.tb00552.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Determinations of serum folate and cobalamin concentrations in dogs have proved of considerable value for the identification and characterisation of chronic small intestinal disorders, but the microbiological assays used are time-consuming and technically demanding. Dual isotope radioassays are more convenient and have been developed for the determination of folate and cobalamin in human beings. This study has evaluated such an assay for the determination of serum folate and cobalamin concentrations in dogs by direct comparison with microbiological assays used previously. Assays were performed on samples from 77 dogs, including controls and animals with confirmed or suspected chronic small intestinal disease. Regression analysis demonstrated a significant relationship between the two assays for serum folate concentrations (R = 0.85; P = 0.0001) and a definite trend for radioassay to give lower results than bioassay. There was also a significant relationship between bioassay and radioassay data for serum cobalamin (R = 0.91; P = 0.0001) with comparable absolute values for these two assays. Radioassay of serum samples from 31 clinically healthy dogs gave control ranges of 3.7 to 8.8-mu-g/litre for folate and 205 to 490 ng/litre for cobalamin. These ranges were similar to those calculated by comparison with the established ranges for bioassay using regression analysis, which predicted ranges of 4.4 to 8.4-mu-g/litre and 217 to 398 ng/litre for radioassay of serum folate and cobalamin, respectively. These data indicate that a dual isotope radioassay of serum folate and cobalamin may be used for dogs, and emphasise the need for laboratories to validate and establish their own control ranges for different assays.
引用
收藏
页码:221 / 224
页数:4
相关论文
共 50 条
  • [21] RADIOASSAY OF SERUM FOLATE, WITH USE OF A SERUM BLANK AND NONDIALYZED MILK AS FOLATE BINDER
    TAUDDIN, M
    GARDYNA, HA
    CLINICAL CHEMISTRY, 1973, 19 (01) : 125 - 126
  • [22] Effect of withholding food on serum concentrations of cobalamin, folate, trypsin-like immunoreactivity, and pancreatic lipase immunoreactivity in healthy dogs
    Saver, Alexander T.
    Steiner, Jorg M.
    Hetzel, Scott J.
    Lidbury, Jonathan A.
    Suchodolski, Jan S.
    Pritchard, Jessica C.
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 2021, 82 (05) : 367 - 373
  • [23] RADIOASSAY FOR SERUM AND RED-CELL FOLATE
    LONGO, DL
    HERBERT, V
    JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 1976, 87 (01): : 138 - 151
  • [24] Serum cobalamin and methylmalonic acid concentrations in dogs with chronic gastrointestinal disease
    Berghoff, Nora
    Parnell, Nolie K.
    Hill, Steve L.
    Suchodolski, Jan S.
    Steiner, Joerg M.
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 2013, 74 (01) : 84 - 89
  • [25] SERUM COBALAMIN AND METHYLMALONIC ACID CONCENTRATIONS IN DOGS WITH CHRONIC GASTROINTESTINAL DISEASE
    Berghoff, N.
    Suchodolski, J. S.
    Steiner, J. M.
    JOURNAL OF VETERINARY INTERNAL MEDICINE, 2012, 26 (03) : 771 - 772
  • [26] DETERMINATION OF SERUM FOLATE LEVELS - COMPARISON OF 4 COMMERCIALLY AVAILABLE IODINATED RADIOASSAY KITS
    KAMPA, IS
    HUNDERTMARK, JM
    CLINICAL CHEMISTRY, 1976, 22 (07) : 1227 - 1227
  • [27] Effects of oral cobalamin supplementation on serum cobalamin concentrations in dogs with exocrine pancreatic insufficiency: A pilot study
    Toresson, Linda
    Steiner, Joerg M.
    Spodsberg, Eva
    Olmedal, Gunilla
    Suchodolski, Jan S.
    Lidbury, Jonathan A.
    Spillmann, Thomas
    VETERINARY JOURNAL, 2021, 269
  • [28] SERUM COBALAMIN AND FOLATE CONCENTRATIONS AS INDICATORS OF GASTROINTESTINAL DISEASE IN KILLER WHALES (ORCINUS ORCA)
    Tang, Karisa N.
    Nollens, Hendrik H.
    Robeck, Todd R.
    Schmitt, Todd L.
    JOURNAL OF ZOO AND WILDLIFE MEDICINE, 2018, 49 (03) : 564 - 572
  • [29] The relationship between serum cobalamin concentration and mean red cell volume at varying concentrations of serum folate
    Metz, J
    McNeil, AR
    Levin, M
    CLINICAL AND LABORATORY HAEMATOLOGY, 2004, 26 (05): : 323 - 325
  • [30] Serum Cobalamin and Folate Concentrations in Common Marmosets (Callithrix jacchus) with Chronic Lymphocytic Enteritis
    Parambeth, Joseph Cyrus
    Ross, Corinna N.
    Miller, Andrew D.
    Austad, Steven N.
    Lidbury, Jonathan A.
    Suchodolski, Jan S.
    Steiner, Jorg M.
    COMPARATIVE MEDICINE, 2019, 69 (02) : 135 - 143