DIRECT MEASUREMENT OF INTRACELLULAR PRESSURE

被引：29

作者：

KELLY, SM ^{[1
]}

MACKLEM, PT ^{[1
]}

机构：

[1] ROYAL VICTORIA HOSP,MONTREAL H3A 1A1,QUEBEC,CANADA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 260卷 / 03期

关键词：

CELL MECHANICS; MICROPRESSURE; OSMOTIC SWELLING; XENOPUS-LAEVIS OOCYTES;

D O I：

10.1152/ajpcell.1991.260.3.C652

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The feasibility of using the servo-null technique for direct measurement of intracellular pressure (P(in)) was investigated. A large cell, the Xenopus laevis oocyte, was chosen for study, and it was established that P(in) obtained with this method was both stable and accurate in these cells. Median resting P(in) in oocytes was found to be 0.27 cmH2O, range 0.14-0.61 cmH2O. During osmotic swelling P(in) increased, in a nonlinear fashion, to a value of 4.11 cmH2O, range 2.61-8.91 cmH2O, with increases in cell volume (V(c)) of 24 +/- 3% (SE). This technique may be of use in the study of cellular mechanics.

引用

页码：C652 / C657

页数：6

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