The role of glutamate in swim initiation in the medicinal leech

被引:20
|
作者
Thorogood, Maria Stella E. [1 ]
Brodfuehrer, Peter D. [1 ]
机构
[1] Bryn Mawr Coll, Dept Biol, Bryn Mawr, PA 19010 USA
关键词
glutamate; non-NMDA; leech (Hirudo medicinalis); swimming;
D O I
10.1007/BF02211024
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Antagonists were used to investigate the role of the excitatory amino acid, L-glutamate, in the swim motor program of Hirudo medicinalis. In previous experiments, focal application of L-glutamate or its non-NMDA agonists onto either the segmental swim-gating interneuron (cell 204) or the serotonergic Retzius cell resulted in prolonged excitation of the two cells and often in fictive swimming. Since brief stimulation of the subesophageal trigger interneuron (cell Trl) evoked a similar response, we investigated the role of glutamate at these synapses. Kynurenic acid and two non-NMDA antagonists, 6,7-dinitroquinoxaline-2,3-dione (DNQX) and Joro spider toxin, effectively suppressed (1) the sustained activation of cell 204 and the Retzius cell following cell Trl stimulation and (2) the mono synaptic connection from cell Trl to cell 204 and the Retzius cell, but did not block spontaneous or DP nerve-activated swimming. Other glutamate blockers, including gamma-D-glutamylaminomethyl sulfonic acid, L(+)-2-amino-3-phosphonoproprionic acid and 2-amino-5-phosphonopentanoic acid, were ineffective. DNQX also blocked both indirect excitation of cell 204 and direct depolarization of cell Trl in response to mechanosensory P cell stimulation. Our findings show the involvement of non-NMDA receptors in activating the swim motor program at two levels: (1) P cell input to cell Trl and (2) cell Trl input to cell 204, and reveal an essential role for glutamate in swim initiation via the cell Trl pathway.
引用
收藏
页码:223 / 233
页数:11
相关论文
共 50 条
  • [31] Role of the extracellular matrix during the postembryonic development of the medicinal leech Hirudo verbana
    Pulze, L.
    Baranzini, N.
    Acquati, F.
    Grimaldi, A.
    ISJ-INVERTEBRATE SURVIVAL JOURNAL, 2023, 20 : 12 - 12
  • [32] HABITUATION OF THE SHORTENING REFLEX IN THE MEDICINAL LEECH
    LOCKERY, SR
    RAWLINS, JNP
    GRAY, JA
    BEHAVIORAL NEUROSCIENCE, 1985, 99 (02) : 333 - 341
  • [33] IDENTIFICATION OF RFAMIDE NEUROPEPTIDES IN THE MEDICINAL LEECH
    EVANS, BD
    POHL, J
    KARTSONIS, NA
    CALABRESE, RL
    PEPTIDES, 1991, 12 (05) : 897 - 908
  • [34] HABITUATION OF SWIMMING ACTIVITY IN THE MEDICINAL LEECH
    DEBSKI, EA
    FRIESEN, WO
    JOURNAL OF EXPERIMENTAL BIOLOGY, 1985, 116 (MAY): : 169 - 188
  • [35] Bleeding due to a medicinal leech bite
    Ikizceli, I
    Avsarogullari, L
    Sözüer, E
    Yürümez, Y
    Akdur, O
    EMERGENCY MEDICINE JOURNAL, 2005, 22 (06) : 458 - 460
  • [36] Scientometric analysis of medicinal leech therapy
    Senel, Engin
    Ozkan, Aysegul Taylan
    Mumcuoglu, Kosta Y.
    JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE, 2020, 11 (04) : 534 - 538
  • [37] ON THE TERMINATION OF INGESTIVE BEHAVIOR BY THE MEDICINAL LEECH
    LENT, CM
    DICKINSON, MH
    JOURNAL OF EXPERIMENTAL BIOLOGY, 1987, 131 : 1 - 15
  • [38] Medicinal leech in head and neck reconstruction
    Irish, JC
    Gullane, PJ
    Mulholland, S
    Neligan, PC
    JOURNAL OF OTOLARYNGOLOGY, 2000, 29 (05): : 327 - 332
  • [39] Medicinal leech fixation in precarious locations
    Bank, Jonathan
    Zilinsky, Yizhak
    Haik, Joseph
    Winkler, Eyal
    Goldan, Oren
    JOURNAL OF RECONSTRUCTIVE MICROSURGERY, 2008, 24 (01) : 67 - 68
  • [40] AN HISTORICAL SURVEY OF ILLUSTRATIONS OF THE MEDICINAL LEECH
    PAYTON, BW
    JOURNAL OF AUDIOVISUAL MEDIA IN MEDICINE, 1984, 7 (03): : 105 - 112