EFFECT OF UV-IRRADIATION ON ROTAVIRUS

被引：0
|
作者：
SMIRNOV, YA [1 ]
KAPITULETS, SP [1 ]
AMITINA, NN [1 ]
GINEVSKAYA, VA [1 ]
KAVERIN, NV [1 ]
机构：
[1] MOSCOW POLIOMYELITIS & VIRAL ENCEPHALITIDES INST,MOSCOW 142782,USSR
来源：
ACTA VIROLOGICA | 1991年 / 35卷 / 01期
关键词：
UV-IRRADIATION; ROTAVIRUS; INACTIVATION; URACIL DIMERS; RNA-PROTEIN LINKAGES;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The effect of UV-irradiation on SAII rotavirus infectivity was followed. The time course of infectivity inactivation in general showed an one-hit pattern. Two basic effects of UV-irradiation on virus particles were investigated: the phenomenon of RNA-protein linkages and the formation of uracil dimers. To determine the number of uridine dimers, H-3-uridine labelled purified rotavirus was exposed to UV-irradiation, subsequently the RNA was extracted and analyzed by ascending paper chromatography. Formation of photodimers was found to be an important mechanism of rotavirus inactivation at conventional UV-irradiation; the RNA-protein linkages were registered at high irradiation dosis only.
引用
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页码：1 / 6
页数：6
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