EFFECT OF UV-IRRADIATION ON ROTAVIRUS

被引：0

作者：

SMIRNOV, YA ^{[1
]}

KAPITULETS, SP ^{[1
]}

AMITINA, NN ^{[1
]}

GINEVSKAYA, VA ^{[1
]}

KAVERIN, NV ^{[1
]}

机构：

[1] MOSCOW POLIOMYELITIS & VIRAL ENCEPHALITIDES INST,MOSCOW 142782,USSR

来源：

ACTA VIROLOGICA | 1991年 / 35卷 / 01期

关键词：

UV-IRRADIATION; ROTAVIRUS; INACTIVATION; URACIL DIMERS; RNA-PROTEIN LINKAGES;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effect of UV-irradiation on SAII rotavirus infectivity was followed. The time course of infectivity inactivation in general showed an one-hit pattern. Two basic effects of UV-irradiation on virus particles were investigated: the phenomenon of RNA-protein linkages and the formation of uracil dimers. To determine the number of uridine dimers, H-3-uridine labelled purified rotavirus was exposed to UV-irradiation, subsequently the RNA was extracted and analyzed by ascending paper chromatography. Formation of photodimers was found to be an important mechanism of rotavirus inactivation at conventional UV-irradiation; the RNA-protein linkages were registered at high irradiation dosis only.

引用

页码：1 / 6

页数：6

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