DETERMINATION OF AMIDEPIN IN HUMAN PLASMA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION

被引：1
|
作者：
STEHLIK, P
HOUBOVA, H
机构：
[1] Research Institute for Pharmacy and Biochemistry, 130 60 Prague 3
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期
关键词：
D O I：
10.1016/0378-4347(91)80413-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An isocratic reversed-phase high-performance liquid chromatographic method for the determination of amidepin has been developed. The method is based on the extraction of alkaline plasma with diethyl ether-dichloromethane, and the injection into the Supelcosil LC-18 column of the evaporated and reconstituted organic phase. After separation, detection is carried out by a fluorescence detector (excitation at 195 nm with no filter). The limit of detection is 10 ng/ml of plasma. The mean coefficient of variation is 12%. The plasma levels after oral administration and after intravenous administration are shown.
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页码：477 / 481
页数：5
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