QUANTIFICATION OF PATHOGENIC MARINE VIBRIO USING MEMBRANE-FILTER TECHNIQUE

A rapid and simple methodology based on a membrane filter technique was designed to detect and quantify marine vibrios involved in fish and shellfish diseases, as well as human gastrointestinal disorders. By placing the membrane in TCBS medium and incubating it at 40-42 degrees C, it was possible to differentiate between pathogenic and non-pathogenic vibrios. A presumptive identification was also done by growing cultures at different salinities and by using a few biochemical tests.