Bacterial aspartate kinase-like activity in human platelet

被引:0
|
作者
ArenasDiaz, G [1 ]
Mercado, LA [1 ]
Marshall, SH [1 ]
机构
[1] PONTIFICIA UNIV CATOLICA VALPARAISO, FAC CIENCIAS BASICAS & MATEMAT, INST BIOL, GENET MOLEC LAB, VALPARAISO, CHILE
关键词
aspartate kinase; human platelets; immunodetection; in vitro phosphorylation;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
One form of a group of enzymes known as aspartate kinases, primarily reported in prokaryotes and plants, might also exist in animal cells. Were we report the immunodetection of an aspartate kinase-like activity in human platelets using antibodies against the pure form of the enzyme purified from Escherichia coli. Moreover, the enrichment of platelet extracts with the bacterial kinase results in the phosphorylation of discrete forms mainly of membrane-bound endogenous polypeptides.
引用
收藏
页码:461 / 465
页数:5
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