HIGHLY SENSITIVE AND HOMOGENEOUS METHOD FOR THE DETERMINATION OF ANTIBODIES AND ANTIGENS BY MEANS OF LIPOSOMES, MONOCLONAL-ANTIBODIES AND COMPLEMENT

被引：0
|
作者：
SKOPINSKAYA, SN
TAMULEVICH, YA
YARKOV, SP
ZLOBIN, VN
KALININ, YT
机构：
来源：
ZHURNAL MIKROBIOLOGII EPIDEMIOLOGII I IMMUNOBIOLOGII | 1993年 / 01期
关键词：
D O I：
暂无
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A simple and sensitive method for the determination of Francisella tularensis antigen and antibodies to this antigen by means of the complement lysis of liposomes sensitized with F. tularensis lipopolysaccharide (LPS) is proposed. The possibility of participation of monoclonal antibodies (McAb) in the complement-dependent lysis of liposomes has been studied. The method permits the detection of 50-100 ng/ml of soluble LPS antigen in the presence of antiserum or McAb IgG F-B11-x and of 50-10 ng/ml of it in the presence of IgG F-B11-x and anti-IgG within 90 minutes.
引用
收藏
页码：77 / 82
页数：6
相关论文
共 50 条
  • [21] MONOCLONAL-ANTIBODIES TO MYELOID DIFFERENTIATION ANTIGENS
    BRADLEY, JG
    OCONNOR, R
    MEAGHER, LC
    COTTER, TG
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1986, 14 (02) : 483 - 484
  • [22] MONOCLONAL-ANTIBODIES AGAINST SPERMATOZOAL ANTIGENS
    PAUL, S
    BAUKLOH, V
    METTLER, L
    AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1982, 2 (05): : 274 - 274
  • [23] MONOCLONAL-ANTIBODIES TO RAT RENAL ANTIGENS
    SHIMIZU, F
    ORIKASA, M
    SATO, K
    OITE, T
    IMMUNOLOGY, 1984, 52 (02) : 319 - 323
  • [24] MONOCLONAL-ANTIBODIES AGAINST BRAIN ANTIGENS
    ADINOLFI, M
    BROWN, S
    DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY, 1983, 25 (05): : 651 - 654
  • [25] MONOCLONAL-ANTIBODIES TO HUMAN ALPHAFETOPROTEIN IN NOVEL, HIGHLY SENSITIVE IMMUNOASSAYS
    RUOSLAHTI, E
    UOTILA, M
    ENGVALL, E
    ONCODEVELOPMENTAL BIOLOGY AND MEDICINE, 1981, 2 (05): : P32 - P32
  • [26] DETERMINATION OF MEMBRANE-ANTIGENS BY A COVALENT CROSS-LINKING METHOD WITH MONOCLONAL-ANTIBODIES
    HAMADA, H
    TSURUO, T
    ANALYTICAL BIOCHEMISTRY, 1987, 160 (02) : 483 - 488
  • [27] A HIGHLY SENSITIVE AND CONVENIENT METHOD TO DETECT MONOCLONAL-ANTIBODIES AGAINST ACETYLCHOLINE-RECEPTORS
    YAMASHITA, Y
    SUGIYAMA, H
    JOURNAL OF BIOCHEMISTRY, 1983, 93 (06): : 1709 - 1712
  • [28] THE CHARACTERIZATION OF MONOCLONAL-ANTIBODIES DIRECTED AGAINST CARBOHYDRATE EPITOPES BY IMMUNOCHEMICAL MEANS - THE IMPORTANCE OF SUCH MONOCLONAL-ANTIBODIES FOR THE RECOGNITION OF BLOOD-GROUP ANTIGENS
    MESSETER, L
    LOW, K
    LUNDBLAD, A
    BIOTECHNOLOGY IN BLOOD TRANSFUSION, 1988, 21 : 65 - 71
  • [29] MONOCLONAL-ANTIBODIES FOR CARCINOEMBRYONIC ANTIGEN AND RELATED ANTIGENS AS A MODEL SYSTEM - A SYSTEMATIC-APPROACH FOR THE DETERMINATION OF EPITOPE SPECIFICITIES OF MONOCLONAL-ANTIBODIES
    WAGENER, C
    YANG, YHJ
    CRAWFORD, FG
    SHIVELY, JE
    JOURNAL OF IMMUNOLOGY, 1983, 130 (05): : 2308 - 2315
  • [30] OPTIMIZATION OF THE COVALENT BINDING OF MONOCLONAL-ANTIBODIES TO LIPOSOMES
    WECKENMANN, HP
    MATZKU, S
    STRICKER, H
    ARZNEIMITTEL-FORSCHUNG/DRUG RESEARCH, 1988, 38-2 (11): : 1556 - 1567
12345