LIGHT-HARVESTING COMPLEXES OF BROWN-ALGAE - BIOCHEMICAL-CHARACTERIZATION AND IMMUNOLOGICAL RELATIONSHIPS

被引:41
|
作者
PASSAQUET, C
THOMAS, JC
CARON, L
HAUSWIRTH, N
PUEL, F
BERKALOFF, C
机构
[1] Laboratoire des Biomembranes et Surfaces Cellulaires Vègètales, UA CNRS DO311, Ecole Normale Supèrieure
关键词
BROWN ALGAE; FUCOXANTHIN; LIGHT-HARVESTING COMPLEX; POLYCLONAL ANTIBODY;
D O I
10.1016/0014-5793(91)80195-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pigment composition of the light-harvesting complexes isolated from several brown algae belonging to different orders has been analysed by reverse-phase HPLC. Relative to whole chloroplasts, they were markedly enriched in Chl c, fucoxanthin and violaxanthin and conversely depleted in Chl a. The relative molar proportions of the 4 main pigments (Chl a/Chl c/fucoxanthin/violaxanthin) ranged from 100:18:76:6 to 100:30:107:17. The protein moiety of LH complexes of all the species studied were composed of one or two main polypeptide components in the range of 19-22 kDa. These polypeptide subunits were arranged in polymeric particles about 240 kDa in Laminaria saccharina. A polyclonal antibody raised against the LH polypeptide of Fucus serratus has been tested on LH apoproteins of other Chromophytes and Chlorophytes. Phylogenic inplications of these results are discussed.
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页码:21 / 26
页数:6
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