DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE AND NEBIVOLOL IN RAW AND TABLET FORMULATION

The main objective of this study is to determine Amlodipine and Nebivolol in tablet formulations using reverse phase high pressure liquid chromatography method. The HPLC separation was carried out on Thermo hypersil - keystone C18 (250 x 4.6mm, 0.5 mu) column in isocratic mode, using a mobile phase mixture comprising a 67: 33(% v/v) of Acetonitrile: phosphate buffer and UV detection at 280 nm. The percentage recoveries 99.70 % and 99.62% for Amlodipine and Nebivolol respectively. The proposed method of RP-HPLC shows good separation of Amlodipine and Nebivolol, were retention time was found to 2.1min for Amlodipine and 5.3 min for Nebivolol, which show very less time consuming analysis. No previous method of analysis shows good separation at less retention time and less cost. Linearity range for Amlodipine and Nebivolol were 10-50 mu g/ml, 10-50 mu g/ml. The developed method of analysis can be applied for the Amlodipine and Nebivolol pharmaceutical dosage form.