SYNTHESIS, MEMBRANE INSERTION AND GLYCOSYLATION OF THYROGLOBULIN IN A COMPLETELY HETEROLOGOUS SYSTEM

被引：3

作者：

EGGO, MC ^{[1
]}

BURROW, GN ^{[1
]}

机构：

[1] UNIV TORONTO, DEPT MED, TORONTO, ONTARIO, CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 04期

关键词：

D O I：

10.1016/0006-291X(79)90226-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thyroglobulin was synthesized, inserted into membranes and glycosylated in a completely heterologous, reconstituted system consisting of a protein synthesizing extract, stripped dog pancreas microsomal membranes and calf thyroid RNA. There is now considerable evidence to support the hypothesis that secreted glycoproteins are synthesized on polysomes which become membrane-bound following synthesis of an N-terminal hydrophobic signal sequence. This signal presequence was found on more than 20 nascent proteins destined for secretion, ovalbumin being the only exception.

引用

页码：1069 / 1076

页数：8

共 50 条

[1] INVITRO SYNTHESIS OF A THYROGLOBULIN PRECURSOR BY PORCINE THYROID MEMBRANE-BOUND RIBOSOMES IN A HETEROLOGOUS CELL-FREE SYSTEM
DILAURO, R
METAFORA, S
CONSIGLIO, E
GENTILE, G
VARRONE, S
JOURNAL OF BIOLOGICAL CHEMISTRY, 1975, 250 (09) : 3267 - 3272
[2] INVITRO SYNTHESIS, GLYCOSYLATION, AND MEMBRANE INSERTION OF INFLUENZA-VIRUS HEMAGGLUTININ
ELDER, KT
BYE, JM
SKEHEL, JJ
WATERFIELD, MD
SMITH, AE
VIROLOGY, 1979, 95 (02) : 343 - 350
[3] MEMBRANE ASSEMBLY INVITRO - SYNTHESIS, GLYCOSYLATION, AND ASYMMETRIC INSERTION OF A TRANSMEMBRANE PROTEIN
KATZ, FN
ROTHMAN, JE
LINGAPPA, VR
BLOBEL, G
LODISH, HF
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) : 3278 - 3282
[4] SYNTHESIS OF LARGE THYROGLOBULIN PEPTIDES IN 3 HETEROLOGOUS SYSTEMS
EGGO, M
BURROW, GN
ANNALES D ENDOCRINOLOGIE, 1978, 39 (05) : A8 - A8
[5] INVITRO SYNTHESIS, GLYCOSYLATION, AND MEMBRANE INSERTION OF THE 4 SUBUNITS OF TORPEDO ACETYLCHOLINE-RECEPTOR
ANDERSON, DJ
BLOBEL, G
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (09): : 5598 - 5602
[6] Membrane insertion, glycosylation, and oligomerization of inositol trisphosphate receptors in a cell-free translation system
Joseph, SK
Boehning, D
Pierson, S
Nicchitta, CV
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (03) : 1579 - 1588
[7] Targeting and insertion of heterologous membrane proteins in E-coli
Raine, A
Ullers, R
Pavlov, M
Luirink, J
Wikberg, JES
Ehrenberg, M
BIOCHIMIE, 2003, 85 (07) : 659 - 668
[8] Membrane insertion and glycosylation of truncation mutants of bovine opsin.
Heymann, JAW
Subramaniam, S
BIOPHYSICAL JOURNAL, 1996, 70 (02) : WP362 - WP362
[9] SYNCHRONIZED TRANSMEMBRANE INSERTION AND GLYCOSYLATION OF A NASCENT MEMBRANE-PROTEIN
ROTHMAN, JE
LODISH, HF
NATURE, 1977, 269 (5631) : 775 - 780
[10] GLYCOSYLATION IS NOT NECESSARY FOR MEMBRANE INSERTION AND CLEAVAGE OF SEMLIKI-FOREST VIRUS MEMBRANE PROTEINS
GAROFF, H
SCHWARZ, RT
NATURE, 1978, 274 (5670) : 487 - 490

← 1 2 3 4 5 →