CONSTITUTIVE EXPRESSION OF HEAT-SHOCK PROTEIN-90 (HSP90) IN NEURONS OF THE RAT-BRAIN

被引：31
|
作者：
GASS, P [1 ]
SCHRODER, H [1 ]
PRIOR, P [1 ]
KIESSLING, M [1 ]
机构：
[1] UNIV COLOGNE,DEPT ANAT,COLOGNE,GERMANY
来源：
NEUROSCIENCE LETTERS | 1994年 / 182卷 / 02期
关键词：
HSP90; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING; IMMUNE-ELECTRON MICROSCOPY;
D O I：
10.1016/0304-3940(94)90794-3
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Immunoblot analysis, immunocytochemistry and immune-electron microscopy were employed to study the expression of HSP90 protein in the adult rat brain, using a specific polyclonal antiserum. Immunoblot analysis demonstrated equal levels of HSP90 in microdissected extracts from hippocampus, cortex, striatum and cerebellum. Immunocytochemistry and immune-electron microscopy provided evidence that HSP90 is markedly expressed thoughout all neuronal subpopulations of the CNS but not in non-neuronal cells except ependyma and choroid plexus. At the ultrastructural level, HSP90 immunoreactivity was predominantly found in perikarya but to a lesser extent also in dendrites and nuclei. The constitutive expression of HSP90 in widespread neuronal cell populations suggests a functional role in the physiological molecular program of CNS neurons.
引用
收藏
页码：188 / 192
页数：5
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