PHOTOMETRIC OR FLUOROMETRIC ASSAY OF CATHEPSIN-B, CATHEPSIN-L AND CATHEPSIN-H AND PAPAIN USING SUBSTRATES WITH AN AMINOTRIFLUOROMETHYLCOUMARIN LEAVING GROUP

被引：68
|
作者：
TCHOUPE, JR
MOREAU, T
GAUTHIER, F
BIETH, JG
机构：
[1] UNIV LOUIS PASTEUR STRASBOURG,INSERM,UNITE 237,F-67400 ILLKIRCH GRAFFENS,FRANCE
[2] UNIV TOURS,CNRS,URA 1334,PROT LIQUIDES BIOL LAB,F-37041 TOURS,FRANCE
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1076卷 / 01期
关键词：
CATHEPSIN; FLUOROMETRY; PAPAIN; CYSTEINE PROTEINASE; LYSOSOMAL ENZYME;
D O I：
10.1016/0167-4838(91)90232-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
N-trifluoromethylcoumarinylamide derivatives of benzyloxycarbonyl-Arg-Arg, benzyloxycarbonyl-Phe-Arg and Arg are convenient chromogenic and fluorogenic substrates of cathepsin B, L and H, respectively. Benzyloxycarbonyl-Phe-Arg-N-trifluoromethylcoumarinylamide is also a highly sensitive substrate for papain.
引用
收藏
页码：149 / 151
页数：3
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