PHOTOMETRIC OR FLUOROMETRIC ASSAY OF CATHEPSIN-B, CATHEPSIN-L AND CATHEPSIN-H AND PAPAIN USING SUBSTRATES WITH AN AMINOTRIFLUOROMETHYLCOUMARIN LEAVING GROUP

被引：68
|
作者：
TCHOUPE, JR
MOREAU, T
GAUTHIER, F
BIETH, JG
机构：
[1] UNIV LOUIS PASTEUR STRASBOURG,INSERM,UNITE 237,F-67400 ILLKIRCH GRAFFENS,FRANCE
[2] UNIV TOURS,CNRS,URA 1334,PROT LIQUIDES BIOL LAB,F-37041 TOURS,FRANCE
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1076卷 / 01期
关键词：
CATHEPSIN; FLUOROMETRY; PAPAIN; CYSTEINE PROTEINASE; LYSOSOMAL ENZYME;
D O I：
10.1016/0167-4838(91)90232-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
N-trifluoromethylcoumarinylamide derivatives of benzyloxycarbonyl-Arg-Arg, benzyloxycarbonyl-Phe-Arg and Arg are convenient chromogenic and fluorogenic substrates of cathepsin B, L and H, respectively. Benzyloxycarbonyl-Phe-Arg-N-trifluoromethylcoumarinylamide is also a highly sensitive substrate for papain.
引用
收藏
页码：149 / 151
页数：3
相关论文
共 50 条
  • [1] CATHEPSIN-B, CATHEPSIN-H, AND CATHEPSIN-L
    BARRETT, AJ
    KIRSCHKE, H
    [J]. METHODS IN ENZYMOLOGY, 1981, 80 : 535 - 561
  • [2] PRIMARY STRUCTURE OF BOVINE CATHEPSIN-S - COMPARISON TO CATHEPSIN-L, CATHEPSIN-H, CATHEPSIN-B AND PAPAIN
    WIEDERANDERS, B
    BROEMME, D
    KIRSCHKE, H
    KALKKINEN, N
    RINNE, A
    PAQUETTE, T
    TOOTHMAN, P
    [J]. FEBS LETTERS, 1991, 286 (1-2) : 189 - 192
  • [3] DIFFERENT IMMUNOLOCALIZATIONS OF CATHEPSIN-B, CATHEPSIN-H, AND CATHEPSIN-L IN THE LIVER
    II, K
    HIZAWA, K
    KOMINAMI, E
    BANDO, Y
    KATUNUMA, N
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1985, 33 (11) : 1173 - 1175
  • [4] CATHEPSIN-B, CATHEPSIN-H AND CATHEPSIN-L IN HUMAN BREAST-CARCINOMA
    GABRIJELCIC, D
    SVETIC, B
    SPAIC, D
    SKRK, J
    BUDIHNA, M
    DOLENC, I
    POPOVIC, T
    COTIC, V
    TURK, V
    [J]. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 1992, 30 (02): : 69 - 74
  • [5] ASSAY PROCEDURES FOR CATHEPSIN-B, CATHEPSIN-H AND CATHEPSIN-L ACTIVITIES IN RAT-TISSUE HOMOGENATES
    HIGASHI, T
    HASHIMOTO, M
    WATANABE, M
    YAMAUCHI, Y
    FUJIWARA, M
    NAKATSUKASA, H
    KOBAYASHI, M
    WATANABE, A
    NAGASHIMA, H
    [J]. ACTA MEDICA OKAYAMA, 1986, 40 (01) : 27 - 32
  • [6] IMMUNOCYTOCHEMICAL LOCALIZATION OF CATHEPSIN-B, CATHEPSIN-H, AND CATHEPSIN-L IN THE RAT GASTRODUODENAL MUCOSA
    FURUHASHI, M
    NAKAHARA, A
    FUKUTOMI, H
    KOMINAMI, E
    GRUBE, D
    UCHIYAMA, Y
    [J]. HISTOCHEMISTRY, 1991, 95 (03) : 231 - 239
  • [7] SEQUENCE HOMOLOGIES, HYDROPHOBIC PROFILES AND SECONDARY STRUCTURES OF CATHEPSIN-B, CATHEPSIN-H AND CATHEPSIN-L - COMPARISON WITH PAPAIN AND ACTINIDIN
    DUFOUR, E
    [J]. BIOCHIMIE, 1988, 70 (10) : 1335 - 1342
  • [8] RAT-LIVER THIOL PROTEINASES - CATHEPSIN-B, CATHEPSIN-H AND CATHEPSIN-L
    KATUNUMA, N
    TOWATARI, T
    KOMINAMI, E
    HASHIDA, S
    TAKIO, K
    TITANI, K
    [J]. ACTA BIOLOGICA ET MEDICA GERMANICA, 1981, 40 (10-1) : 1419 - 1425
  • [9] IMMUNOCHEMICAL INVESTIGATION OF THIOL PROTEINASES OF THE BOVINE SPLEEN - CATHEPSIN-B, CATHEPSIN-H, AND CATHEPSIN-L
    LOKSHINA, LA
    TARKHANOVA, IA
    LUBKOVA, ON
    GOLUBEVA, NV
    GUREEVA, TA
    OREKHOVICH, VN
    [J]. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 1983, 96 (10) : 1399 - 1402
  • [10] CATHEPSIN-B, CATHEPSIN-D, CATHEPSIN-H AND CATHEPSIN-L ACTIVITIES IN THE PROCESSING OF DRY-CURED HAM
    TOLDRA, F
    RICO, E
    FLORES, J
    [J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1993, 62 (02) : 157 - 161
12345