DEVELOPMENT OF ANALYTICAL METHODS TO EVALUATE SFH

被引：3

作者：

NAKAI, K ^{[1
]}

ABE, H ^{[1
]}

MATSUDA, N ^{[1
]}

KOBAYASHI, M ^{[1
]}

IKEDA, H ^{[1
]}

SEKIGUCHI, S ^{[1
]}

TSUCHIDA, E ^{[1
]}

机构：

[1] WASEDA UNIV,TOKYO 169,JAPAN

来源：

BIOMATERIALS ARTIFICIAL CELLS AND IMMOBILIZATION BIOTECHNOLOGY | 1992年 / 20卷 / 2-4期

关键词：

D O I：

10.3109/10731199209119667

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40 nm and the ultrafiltration method with a 100kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.

引用

页码：447 / 451

页数：5

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