A CATECHOL 2,3-DIOXYGENASE GENE AS A REPORTER

被引:5
|
作者
SHINDO, T [1 ]
UEDA, H [1 ]
SUZUKI, E [1 ]
NISHIMURA, H [1 ]
机构
[1] UNIV TOKYO, FAC ENGN, DEPT CHEM ENGN, BUNKYO KU, TOKYO 113, JAPAN
关键词
D O I
10.1271/bbb.59.314
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A catechol 2,3-dioxygenase (C23O) gene of Pseudomonas aeuaginosa was expressed under the Simian virus 40 or Rous sarcoma virus promoter in mammalian cells; it was found that the gene could be used as a reporter for the study of gene expression. The C23O gene was a more sensitive reporter than the generally used beta-galactosidase gene.
引用
收藏
页码:314 / 315
页数:2
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