Biophysicochemical evaluation and micropropagation study of Jatropha curcas L. collections for biodiesel production

Jatropha curcas, a member of the Euphorbiaceae family, is an upcoming energy source, which promises to mitigate the energy crisis and environmental pollution. Jatropha curcas oil is looked up in terms of availability and cost and also has several applications and enormous economic benefits. The seed oils of five Jatropha curcas biotypes were screened and evaluated for their physiochemical parameters, viz. oil content (20-43%), biodiesel yield (48-66%), density (. 866-. 969 g/cm(3)), viscosity (50.12-93.79mm(3)/s), iodine value (232.738-457.16 mg/g), free fatty acid (18.847-7.614 mg/g), saponification value (59.2993.79 mg/g), flash point (125-220 degrees C), fire point (155-260 degrees C) and ash content (. 19-. 399%), which were estimated for selection of the elite Jatropha curcas biotype. The best shoot regeneration (60%) was observed in Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (0.5 ppm) and benzyl amino purine (2.0 ppm). Root induction (90%) was successfully obtained in plain MS. Acclimatisation and hardening was quite successful with survival rate of 70%.