WHOLE-CELL PATCH-CLAMP RECORDINGS FROM RESPIRATORY NEURONS IN NEONATAL RAT BRAIN-STEM INVITRO

被引：61
|
作者：
SMITH, JC [1 ]
BALLANYI, K [1 ]
RICHTER, DW [1 ]
机构：
[1] UNIV GOTTINGEN,INST PHYSIOL 2,W-3400 GOTTINGEN,GERMANY
来源：
NEUROSCIENCE LETTERS | 1992年 / 134卷 / 02期
关键词：
BRAIN-STEM SPINAL CORD INVITRO; MEDULLARY RESPIRATORY NEURON; SYNAPTIC CURRENT; VOLTAGE CLAMP; CURRENT CLAMP; NEONATAL RAT;
D O I：
10.1016/0304-3940(92)90504-Z
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Whole-cell recordings were obtained from respiratory neurons by applying patch-clamp techniques in the en bloc medulla of in vitro neonatal rat brainstem-spinal cord preparations. Stable voltage-clamp recordings of excitatory or inhibitory synaptic drive currents and current-clamp recordings of spike discharge of inspiratory and expiratory neurons could be maintained for periods of 1-2 h. Parameters of whole-cell recording, including membrane seal resistances and series resistances, obtained in the en bloc medulla were similar to those obtained in corresponding regions of thin slices where neurons were directly visualized to optimize conditions for whole-cell patch clamp.
引用
收藏
页码：153 / 156
页数：4
相关论文
共 50 条
  • [21] Whole-cell patch-clamp recording and parameters
    Sodikdjon A. Kodirov
    [J]. Biophysical Reviews, 2023, 15 : 257 - 288
  • [22] Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    Cattaneo, Stefano
    Regoni, Maria
    Sassone, Jenny
    Taverna, Stefano
    [J]. BIO-PROTOCOL, 2021, 11 (15):
  • [23] Whole-cell patch-clamp recording and parameters
    Kodirov, Sodikdjon A.
    [J]. BIOPHYSICAL REVIEWS, 2023, 15 (02) : 257 - 288
  • [24] Intracellular recordings of rat rostral ventrolateral medulla (RVLM) neurones by whole-cell patch-clamp technique
    Oshima, N
    Kumagai, H
    Sakata, K
    Nishizawa, M
    Ichikawa, M
    Saruta, T
    [J]. JOURNAL OF HYPERTENSION, 1998, 16 : S169 - S169
  • [25] PATCH-CLAMP AND WHOLE-CELL RECORDING FROM HUMAN OOCYTES
    DEFELICE, LJ
    MAZZANTI, M
    MURNANE, J
    COHEN, J
    [J]. BIOPHYSICAL JOURNAL, 1988, 53 (02) : A547 - A547
  • [26] WHOLE-CELL PATCH-CLAMP RECORDINGS OF THE RESPONSES OF CULTURED RABBIT TRIGEMINAL GANGLION NEURONS TO CAPSAICIN - AN IRRITANT AND NEUROTOXIN
    BAUMANN, TK
    LAMOTTE, RH
    [J]. IN VITRO TOXICOLOGY : NEW DIRECTIONS, 1989, 7 : 57 - 66
  • [27] FLUCTUATION ANALYSIS OF PATCH-CLAMP OR WHOLE-CELL RECORDINGS CONTAINING MANY SINGLE CHANNELS
    CHUNG, SH
    PULFORD, G
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 1993, 50 (03) : 369 - 384
  • [28] Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
    Booker, Sam A.
    Song, Jie
    Vida, Imre
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (91): : e51706
  • [29] WHOLE-CELL PATCH-CLAMP RECORDINGS OF AN NMDA RECEPTOR-MEDIATED SYNAPTIC CURRENT IN RAT HIPPOCAMPAL SLICES
    RANDALL, AD
    SCHOFIELD, JG
    COLLINGRIDGE, GL
    [J]. NEUROSCIENCE LETTERS, 1990, 114 (02) : 191 - 196
  • [30] SIMULTANEOUS WHOLE-CELL PATCH-CLAMP RECORDING AND IMAGING OF SINGLE PYRAMIDAL NEURONS IN RAT HIPPOCAMPAL SLICES
    ALFORD, S
    COLLINGRIDGE, GL
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1991, 435 : P5 - P5
12345