EFFECTS OF ANOXIA ON RAT MIDBRAIN DOPAMINE NEURONS

被引:48
|
作者
MERCURI, NB [1 ]
BONCI, A [1 ]
JOHNSON, SW [1 ]
STRATTA, F [1 ]
CALABRESI, P [1 ]
BERNARDI, G [1 ]
机构
[1] IRCCS, CLIN S LUCIA, I-00173 ROME, ITALY
关键词
D O I
10.1152/jn.1994.71.3.1165
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Dopamine-containing neurons of the rat midbrain were recorded intracellularly in vitro. Anoxia (2-5 min) caused reversible membrane hyperpolarization (4-25 mV), which blocked spontaneous firing of action potentials. Under voltage clamp, anoxia produced an outward current (100-1,000 pA) associated with an increase in the apparent input conductance. 2. The mean reversal potential of the anoxia-induced response at 2.5 and 12.5 mM [K+] was -86 and -66 mV, respectively. 3. The effect of anoxia was not blocked by tetrodotoxin (TTX), saclofen, (-)sulpiride, or strychnine. Superfusate containing low calcium (0.5 mM CaCl2 and 10 mM MgCl2 or 0.5-1 mM CaCl2 and 1 mM CoCl2) or low sodium (25-40% of control) reduced the anoxia-induced outward current. 4. Extracellular barium (0.1-1 mM) blocked the anoxia-induced hyperpolarization/outward current. Other K+ channel blockers (tetraethylammonium, apamin, quinine, and glibenclamide) failed to reduce anoxia-induced current. 5. When the dopamine-containing neurons were loaded with cesium (1-2 mM), anoxia caused a reversible membrane depolarization and a block of the firing activity. This depolarization was voltage dependent; it was decreased or blocked by the hyperpolarization of the membrane. 6. Perfusion of the cells with 0.5-1 mu M TTX did not affect the membrane depolarization/inward current caused by anoxia. These were also present when the cells were treated with the excitatory amino acid receptor antagonists D,L-2-amino-5-phosphono-valerate (APV) (30 mu M) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) (10 mu M). 7. The exposure of the neurons with low-sodium, low-calcium solutions reversibly reduced the depolarizing/inward effects of anoxia. 8. It is concluded that under control condition anoxia hyperpolarizes dopamine-containing neurons. However, when the potassium current is blocked by intracellular cesium, it depolarizes these cells.
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收藏
页码:1165 / 1173
页数:9
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