MEASUREMENT OF THE CLASS-III ANTIDYSRHYTHMIC DRUG, UK-68,798, IN PLASMA BY RADIOIMMUNOASSAY

被引：26
|
作者：
WALKER, DK
AHERNE, GW
ARROWSMITH, JE
CROSS, PE
KAYE, B
SMITH, DA
STOPHER, DA
WILD, W
机构：
[1] GUILDHAY ANTISERA LTD,RIVERSIDE BUSINESS CTR,UNIT 6,GUILDFORD GU1 4UG,SURREY,ENGLAND
[2] PFIZER LTD,CENT RES,DEPT DISCOVERY CHEM,SANDWICH CT13 9NJ,KENT,ENGLAND
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1991年 / 9卷 / 02期
关键词：
CLASS-III ANTIDYSRHYTHMIC DRUG,UK-68,798; RADIOIMMUNOASSAY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I：
10.1016/0731-7085(91)80137-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive radioimmunoassay (RIA) for the specific determination of 1-(4-methanesulphonamidophenoxy)-2-[N-(4-methanesulphonamido-phenethyl)-N-methylamino]ethane (UK-68,798), a novel class III antidysrhythmic agent, in human plasma is described. Specific antisera were raised in sheep using desmesyl-UK-68, 798-succinate-ovalbumin conjugate as the antigenic hapten carrier protein. The antisera produced exhibited high specificity for UK-68, 798 compared with known metabolites from animals, other antidysrhythmic agents and co-administered drugs. Good correlation was found in a comparison of the RIA method with a high-performance liquid chromatography (HPLC) method (r = 0.997) and a 10-fold lower limit of determination was observed for the RIA method compared with the HPLC method (0.05 and 0.5 ng ml-1, respectively). The RIA method was applied to the analysis of UK-68, 798 in plasma obtained from human volunteers receiving the compound.
引用
收藏
页码：141 / 149
页数：9
相关论文
共 50 条
12345