PURIFICATION AND PROPERTIES OF COLLAGENASE FROM CYTOPHAGA SP L43-1 STRAIN

被引：29
|
作者：
SASAGAWA, Y [1 ]
KAMIO, Y [1 ]
MATSUBARA, Y [1 ]
MATSUBARA, Y [1 ]
SUZUKI, K [1 ]
KOJIMA, H [1 ]
IZAKI, K [1 ]
机构：
[1] NIPPI INC, RES INST, ADACHI KU, TOKYO 120, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期
关键词：
D O I：
10.1271/bbb.57.1894
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A collagenolytic bacterial strain was isolated from soil and was identified as Cytophaga sp. It produced several kinds of collagenase and protease. From the supernatant of a culture, a collagenase was purified as a single protein band upon SDS-PAGE and its molecular mass was estimated to be 120 kDa. Collagen and gelatin were good substrates for this enzyme. Beta-casein was cleaved by this enzyme at several sites.
引用
收藏
页码：1894 / 1898
页数：5
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