NEW ASSAY TO DETECT SHEDDING ANTIGEN WITH USE OF ANTIIDIOTYPIC MONOCLONAL-ANTIBODIES

被引：1
|
作者：
TSUJISAKI, M [1 ]
IMAI, K [1 ]
TOKUCHI, S [1 ]
MASUYA, J [1 ]
ISHIDA, T [1 ]
ADACHI, M [1 ]
HINODA, Y [1 ]
HANZAWA, Y [1 ]
YACHI, A [1 ]
机构：
[1] AJINOMOTO CO INC,LIFE SCI LAB,KAWASAKI,KANAGAWA 210,JAPAN
来源：
JOURNAL OF CLINICAL LABORATORY ANALYSIS | 1991年 / 5卷 / 01期
关键词：
ANTIIDIOTYPIC MONOCLONAL ANTIBODY; CARCINOEMBRYONIC ANTIGEN; SHEDDING ANTIGEN; IDIOTYPE MAPPING; IDIOTYPE-ANTI-IDIOTYPE INTERACTION;
D O I：
10.1002/jcla.1860050104
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Anti-idiotypic (Anti-Id) monoclonal antibodies (MoAbs) against anti-carcinoembryonic antigen (CEA) monoclonal antibody 5B3 (IgG1) were prepared and characterized. Five anti-Id MoAbs recognized the private idiotype of MoAb 5B3. Idiotype mapping suggested that MoAb 5B3 had at least three distinct idiotopes at its antigen combining site. Utilizing the inhibition of idiotype-anti-idiotype interaction with use of anti-Id MoAb T4-305 or T4-212, an immunoassay to detect shedding CEA was established. The results of this inhibition assay correlated with those of double determinant immunoassay and inhibition percentages linearly increased in a CEA concentration-dependent manner. Since it requires only one epitope of a given antigen molecule, this assay could be widely used for detection of shedding antigens, including tumor-associated antigens.
引用
收藏
页码：14 / 20
页数：7
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