PHENYLALANYL-TRANSFER RNA-SYNTHETASE FROM THERMUS-THERMOPHILUS CAN ATTACH 2 MOLECULES OF PHENYLALANINE TO TRANSFER RNA(PHE)

被引：19
|
作者：
STEPANOV, VG
MOOR, NA
ANKILOVA, VN
LAVRIK, OI
机构：
[1] Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, the Russian Academy of Sciences, 630090 Novosibirsk
来源：
FEBS LETTERS | 1992年 / 311卷 / 03期
关键词：
PHENYLALANYL-TRANSFER RNA SYNTHETASE; TRANSFER RNA(PHE); TRANSFER RNA AMINOACYLATION;
D O I：
10.1016/0014-5793(92)81099-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phenylalanyl-tRNA synthetase from the extreme thermophilic bacterium Thermus thermophilus can incorporate more than one molecule of phenylalanine into the tRNA(Phe). It is shown that the 'hyperaminoacylated' tRNA(Phe) is the bis-2',3'-O-phenylalanyl-tRNA(Phe), and its formation is typical for the thermophilic enzyme but does not occur for E. coli phenylalanyl-tRNA synthetase under the same conditions.
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页码：192 / 194
页数：3
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