A RAPID SCREEN FOR THE DETECTION OF SPECIFIC DNA-SEQUENCES IN PLANTS

被引：2

作者：

IRVINE, JM ^{[1
]}

OAKES, JV ^{[1
]}

SHEWMAKER, CK ^{[1
]}

CROSSWAY, A ^{[1
]}

机构：

[1] CALGENE INC,1920 5TH ST,DAVIS,CA 95616

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 02期

关键词：

D O I：

10.1016/0735-0651(90)90008-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple and quick method ("wick blot") for detecting the presence of specific DNA sequences i in plants, using radiolabeled DNA probes. The method requires only small amounts of tissue, about 15-25 mg. More than a hundred samples per day can be easily extracted and blotted. It works well on various species and tissues, including leaves, embryos, and callus. The method is ideally suited for screening large numbers of putative transformants, especially populations that have not been screened by prior selection. © 1990.

引用

页码：25 / 31

页数：7

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