Pentacyclic Triterpenoids and Steroids from Voacanga megacarpa

Chromatographic purification of the crude DCM-methanolic extract of the Philippine endemic medicinal plant, Voacanga megacarpa, afforded a 1:1 mixture of lupeol acetate (1) and beta-amyrin acetate (1), and a 1: 1 mixture of stigmasterol (3) and beta-sitosterol (4). The compounds were identified through analysis of their NMR spectroscopic data and by comparison with reported literature data. This is the first report on the isolation and identification of these compounds from V. megacarpa. The crude DCM-methanolic and alkaloid extracts, and fractions (1 and 2) showed moderate inhibitory activity against Mycobacterium tuberculosis H(37)Rv (MIC50 = 64 mu g/mL).