ENHANCED SURFACE-PLASMON RESONANCE INHIBITION TEST (ESPRIT) USING LATEX-PARTICLES

被引：50
|
作者：
SEVERS, AH
SCHASFOORT, RBM
机构：
[1] Department of Microbiology, TNO-Nutrition and Food Research, 3700 AJ Zeist
来源：
BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 7-8期
关键词：
SURFACE PLASMON RESONANCE; SUBMICRON LATEX PARTICLES; HUMAN CHORIONIC GONADOTROPIN; ESPRIT;
D O I：
10.1016/0956-5663(93)80075-Z
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The pregnancy hormone human chorionic gonadotropin (hCG) was used as a model antigen to describe a new assay, the Enhanced Surface Plasmon Resonance Inhibition Test (ESPRIT). It was shown that the introduction of sub-micron latex particles instead of anti-antibodies in an enhancement step improved the sensitivity of the assay by a factor of 30. Latex particles are therefore considered to be versatile tools in the development of new immunochemical assays for the detection of any analyte using SPR immunosensors.
引用
收藏
页码：365 / 370
页数：6
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