ISOLATION, SEQUENCE AND FUNCTIONAL EXPRESSION OF THE MOUSE M4 MUSCARINIC ACETYLCHOLINE-RECEPTOR GENE

被引：15
|
作者：
VANKOPPEN, CJ [1 ]
LENZ, W [1 ]
NATHANSON, NM [1 ]
机构：
[1] UNIV WASHINGTON,DEPT PHARMACOL SJ-30,SEATTLE,WA 98195
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1173卷 / 03期
关键词：
MUSCARINIC RECEPTOR GENE; G-PROTEIN-COUPLED RECEPTOR; ACETYLCHOLINE; NUCLEOTIDE SEQUENCE; (MOUSE);
D O I：
10.1016/0167-4781(93)90135-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Molecular cloning studies have demonstrated the existence in mammals of five genes encoding distinct muscarinic acetylcholine receptors which are heterogeneously expressed in the central nervous system and the target organs of the autonomic nervous system. In order to determine the factors responsible for regulation of receptor expression, we isolated a genomic clone encoding the mouse m4 muscarinic acetylcholine receptor. The gene contains no introns in the coding sequence, and encodes a polypeptide of 479 amino acids that is able to bind various muscarinic ligands with affinities typical for mAChRs and to couple efficiently to inhibition of adenylyl cyclase.
引用
收藏
页码：342 / 344
页数：3
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