WATER-TEST - A SIMPLE METHOD TO ASSESS SPERM-MEMBRANE INTEGRITY

被引：48

作者：

LOMEO, AM ^{[1
]}

GIAMBERSIO, AM ^{[1
]}

机构：

[1] OSPED S CARLO BORROMED,DEPT OBSTET & GYNECOL,VIA SICILIA 19,I-85100 POTENZA,ITALY

来源：

INTERNATIONAL JOURNAL OF ANDROLOGY | 1991年 / 14卷 / 04期

关键词：

EOSIN-Y; HYPOOSMOTIC SWELLING TEST; INFERTILE MEN; WATER-TEST;

D O I：

10.1111/j.1365-2605.1991.tb01093.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

The functional integrity of the membrane of sperm from semen samples collected from 10 fertile men (Group A) and from 50 infertile men (Group B) was assessed by studying the swelling reaction of sperm when suspended in a medium of distilled water ('Water-test'). The results were correlated with routine semen-analysis and with the results of Eosin-Y staining. The mean values for the 'Water-test' were significantly different (P < 0.01) between Groups A and B (84.8 +/- 3.5 versus 70.9 +/- 13.3, respectively). No significant correlations were observed in either Group A or B, between values for the 'Water-test' and values for the sperm count, the percentage of sperm with normal morphology and the percentage of motile sperm. There was a good correlation in both Group A (r = 0.86, P < 0.01) and Group B (r = 0.92, P < 0.01) between values for the 'Water-test' and those for the Eosin-Y test. These results indicate that the 'Water-test' is a simple and reliable test for evaluating sperm membrane integrity.

引用

页码：278 / 282

页数：5

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