MOLECULAR-CLONING OF THE HUMAN ANGIOTENSIN-II TYPE-2 RECEPTOR CDNA

A human adult lung cDNA library was screened and one full-length human angiotensin II type 2 receptor (hAT(2)R) clone was isolated and characterized. The hAT(2)R cDNA clone contains a 1089-base-pair open reading frame which encodes a protein of 363 amino acid residues. The hAT(2)R is approximately 92% identical in sequence to the rat and mouse AT(2)R sequences. Specific binding of [I-125]CGP42112A was demonstrated in membranes from COS-7 cells transiently transfected with the hAT(2)R cDNA. Scatchard analysis and ligand displacement profiles were typical of the AT(2)R. Northern analysis demonstrated that the hAT(2)R mRNA was abundantly expressed in human adult lung and in human fetal kidney. Additionally, the hAT(2)R mRNA was just detectable in human adult heart and aorta. In contrast, the rat AT(2)R mRNA was abundantly expressed in the rat brain and just detectable in the rat lung. (C) 1994 Academic Press, Inc.