PROTEIN TAILORING OF HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR

被引：2
|
作者：
ISHIKAWA, M
OKADA, Y
ISHIKAWA, R
TSUMURA, H
MATSUKI, S
ASANO, K
机构：
[1] Pharmaceutical Laboratory, Kirin Brewery Co., Ltd., Takasaki Gunma, 371
来源：
BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 07期
关键词：
D O I：
10.1007/BF01080138
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recombinant human granulocyte-colony stimulating factor (rhG-CSF) was modified by site-directed mutagenesis and chemical modification in order to improve its pharmacological activity and its thermostability. The mutant rhG-CSF which 17th cysteine was substituted with alanine was chemically modified by activated polyethylene glycol. The chemically modified mutant rhG-CSF greatly increased both its biological activity in vivo and its thermostability. This is a successful example of protein tailoring in which site-directed mutagenesis and chemical modification were used at the same time.
引用
收藏
页码：673 / 678
页数：6
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