PHASE-SELECTIVE ALTERNATING-CURRENT POLAROGRAPHIC ASSAY OF METHOTREXATE IN HUMAN SERUM

被引：10
|
作者：
ORDIERES, AJM [1 ]
GARCIA, AC [1 ]
ALVAREZ, JMF [1 ]
BLANCO, PT [1 ]
机构：
[1] UNIV OVIEDO,DEPT QUIM FIS & ANALIT,E-3071 OVIEDO,SPAIN
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 233卷 / 02期
关键词：
D O I：
10.1016/S0003-2670(00)83489-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The a.c. polarographic behaviour of methotrexate (MTX) was studied and the nature of the process taking place at the dropping mercury electrode was elucidated. Both the molecule and its reduced product appeared to be absorbed at the surface of the electrode. The observed electrochemical behaviour was in close agreement with theoretical predictions for an absorbed species which is reversibly reduced. This permitted a very sensitive and selective determination of MTX in serum samples by phase-selective a.c. polarography. The method, which involves a very simple and rapid previous clean-up procedure, has a limit of detection of 3.0 × 10-7 M in serum and the results compare well with those obtained by liquid chromatography with oxidative amperometric detection (LC-ED) at +1.1 V. Both methods form an interesting alternative to others previously reported for monitoring MTX in cancer patients under treatment with high doses of the drug. © 1990.
引用
收藏
页码：281 / 287
页数：7
相关论文
共 50 条
12345