PHASE-SELECTIVE ALTERNATING-CURRENT POLAROGRAPHIC ASSAY OF METHOTREXATE IN HUMAN SERUM

被引：10

作者：

ORDIERES, AJM ^{[1
]}

GARCIA, AC ^{[1
]}

ALVAREZ, JMF ^{[1
]}

BLANCO, PT ^{[1
]}

机构：

[1] UNIV OVIEDO,DEPT QUIM FIS & ANALIT,E-3071 OVIEDO,SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 233卷 / 02期

关键词：

D O I：

10.1016/S0003-2670(00)83489-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The a.c. polarographic behaviour of methotrexate (MTX) was studied and the nature of the process taking place at the dropping mercury electrode was elucidated. Both the molecule and its reduced product appeared to be absorbed at the surface of the electrode. The observed electrochemical behaviour was in close agreement with theoretical predictions for an absorbed species which is reversibly reduced. This permitted a very sensitive and selective determination of MTX in serum samples by phase-selective a.c. polarography. The method, which involves a very simple and rapid previous clean-up procedure, has a limit of detection of 3.0 × 10-7 M in serum and the results compare well with those obtained by liquid chromatography with oxidative amperometric detection (LC-ED) at +1.1 V. Both methods form an interesting alternative to others previously reported for monitoring MTX in cancer patients under treatment with high doses of the drug. © 1990.

引用

页码：281 / 287

页数：7

共 50 条

[1] PHASE-SELECTIVE INTER-MODULAR ALTERNATING-CURRENT POLAROGRAPHY AND VOLTAMMETRY
BLUTSTEIN, H
BOND, AM
NORRIS, A
[J]. ANALYTICAL CHEMISTRY, 1976, 48 (13) : 1975 - 1979
[2] NEW INSTRUMENTAL APPROACH IN PHASE-SELECTIVE SECOND-HARMONIC ALTERNATING-CURRENT POLAROGRAPHY
BLUTSTEIN, H
BOND, AM
NORRIS, A
[J]. ANALYTICAL CHEMISTRY, 1974, 46 (12) : 1754 - 1758
[3] POLAROGRAPHIC METHODS FOR ALTERNATING-CURRENT
BRYKSIN, IE
SALIKHDZ.RM
[J]. ZAVODSKAYA LABORATORIYA, 1974, 40 (04): : 366 - 370
[4] ALTERNATING-CURRENT LINEAR SWEEP AND STRIPPING VOLTAMMETRY WITH PHASE-SELECTIVE SECOND-HARMONIC DETECTION
BLUTSTEIN, H
BOND, AM
[J]. ANALYTICAL CHEMISTRY, 1974, 46 (11) : 1531 - 1538
[5] PHASE-SELECTIVE ALTERNATING CURRENT POLAROGRAPHY AT STATIONARY ELECTRODES
SMITH, DE
REINMUTH, WH
[J]. ANALYTICAL CHEMISTRY, 1960, 32 (13) : 1892 - 1893
[6] ALTERNATING-CURRENT POLAROGRAPHIC INVESTIGATION OF POLYSACCHARIDES IN DNA
MALFOY, B
REYNAUD, JA
[J]. ANALYTICAL BIOCHEMISTRY, 1978, 84 (01) : 1 - 11
[7] THEORY OF DIGITAL ALTERNATING-CURRENT POLAROGRAPHIC TECHNIQUES
ANDERSON, JE
BOND, AM
[J]. ANALYTICAL CHEMISTRY, 1982, 54 (09) : 1575 - 1578
[8] PHASE-SELECTIVE ALTERNATING-CURRENT ADSORPTIVE STRIPPING VOLTAMMETRY OF FOLIC-ACID ON A MERCURY THIN-FILM ELECTRODE
DELPOZO, JA
GARCIA, AC
ORDIERES, AJM
BLANCO, PT
[J]. ELECTROANALYSIS, 1992, 4 (01) : 87 - 92
[9] PHASE-SELECTIVE ALTERNATING-CURRENT ADSORPTIVE STRIPPING VOLTAMMETRY OF AMINOPTERIN ON A MERCURY THIN-FILM CARBON-FIBER ULTRAMICROELECTRODE
MALONE, MA
GARCIA, AC
BLANCO, PT
SMYTH, MR
[J]. ANALYST, 1993, 118 (06) : 649 - 655
[10] ANALYTICAL APPLICATIONS OF PHASE-SELECTIVE 2ND HARMONIC ALTERNATING-CURRENT POLAROGRAPHY - SIMULTANEOUS DETERMINATION OF INDIUM(III) AND CADMIUM(II)
FAGIOLI, F
DONDI, F
BIGHI, C
[J]. ANNALI DI CHIMICA, 1978, 68 (1-2) : 111 - 121

← 1 2 3 4 5 →