ANTISENSE DNA OLIGONUCLEOTIDES .1. THE USE OF IONSPRAY TANDEM MASS-SPECTROMETRY FOR THE SEQUENCE VERIFICATION OF METHYLPHOSPHONATE OLIGODEOXYRIBONUCLEOTIDES

被引：37

作者：

BAKER, TR ^{[1
]}

KEOUGH, T ^{[1
]}

DOBSON, RLM ^{[1
]}

RILEY, TA ^{[1
]}

HASSELFIELD, JA ^{[1
]}

HESSELBERTH, PE ^{[1
]}

机构：

[1] JBL SCI INC,SAN LUIS OBISPO,CA 93401

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 03期

关键词：

D O I：

10.1002/rcm.1290070305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequences of synthetically prepared methylphosphonate oligodeoxyribonucleotides have been verified using ionspray tandem mass spectrometry with sample introduction via flow injection. The technique involves the use of product-ion scans from multiply protonated (4+ and 5+) precursors. Among the ions detected are several series of fragments of different charge states that indicate the base sequence of the intact molecule. Oligomers as large as 18 bases have been successfully characterized.

引用

页码：190 / 194

页数：5

共 25 条

[21] STUDIES IN NORBORNENE SERIES .1. ELUCIDATION OF STRUCTURE OF NORBORNENE DERIVATIVES BY USE OF NUCLEAR MAGNETIC-RESONANCE SHIFT REAGENT TRISDIPIVALOYLMETHANATOEUROPIUM(III), NUCLEAR OVERHAUSER EFFECT, AND MASS-SPECTROMETRY
BARRACLO.D
OAKLAND, JS
SCHEINMA.F
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1972, (12): : 1500 - &
[22] REVISION OF THE AMINO-ACID-SEQUENCE OF THE SMALLEST BC1-COMPLEX SUBUNIT - USE OF FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY AND MASS-ANALYZED ION KINETIC-ENERGY SPECTRUM ANALYSIS
TERZI, E
BOYOT, P
VANDORSSELAER, A
LUU, B
TRIFILIEFF, E
BIOLOGICAL MASS SPECTROMETRY, 1991, 20 (04) : 232 - 236
[23] MECHANISM OF ACTION OF (2-HALOETHYL)NITROSOUREAS ON DNA - DISCRIMINATION BETWEEN ALTERNATIVE PATHWAYS OF DNA-BASE MODIFICATION BY 1,3-BIS(2-FLUOROETHYL)-1-NITROSOUREA BY USING SPECIFIC DEUTERIUM LABELING AND IDENTIFICATION OF REACTION-PRODUCTS BY HPLC TANDEM MASS-SPECTROMETRY
NAGHIPUR, A
IKONOMOU, MG
KEBARLE, P
LOWN, JW
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (08) : 3178 - 3187
[24] DETERMINATION OF IN-VITRO FORMED DNA-ADDUCTS OF 2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE USING CAPILLARY LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION TANDEM MASS-SPECTROMETRY
RINDGEN, D
TURESKY, RJ
VOUROS, P
CHEMICAL RESEARCH IN TOXICOLOGY, 1995, 8 (08) : 1005 - 1013
[25] 2-STAGE ION-BEAM MASS-SPECTROMETRY .1. APPARATUS AND ITS USE TO STUDY REACTION OF ACETYLIUM (CH3CO+) AND NITRONIUM (NO2+) IONS WITH AROMATIC-COMPOUNDS IN GAS-PHASE
IZOD, TPJ
TEDDER, JM
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 1974, 337 (1610): : 333 - 350

← 1 2 3 →