ANTISENSE DNA OLIGONUCLEOTIDES .1. THE USE OF IONSPRAY TANDEM MASS-SPECTROMETRY FOR THE SEQUENCE VERIFICATION OF METHYLPHOSPHONATE OLIGODEOXYRIBONUCLEOTIDES

被引：37

作者：

BAKER, TR ^{[1
]}

KEOUGH, T ^{[1
]}

DOBSON, RLM ^{[1
]}

RILEY, TA ^{[1
]}

HASSELFIELD, JA ^{[1
]}

HESSELBERTH, PE ^{[1
]}

机构：

[1] JBL SCI INC,SAN LUIS OBISPO,CA 93401

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 03期

关键词：

D O I：

10.1002/rcm.1290070305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequences of synthetically prepared methylphosphonate oligodeoxyribonucleotides have been verified using ionspray tandem mass spectrometry with sample introduction via flow injection. The technique involves the use of product-ion scans from multiply protonated (4+ and 5+) precursors. Among the ions detected are several series of fragments of different charge states that indicate the base sequence of the intact molecule. Oligomers as large as 18 bases have been successfully characterized.

引用

页码：190 / 194

页数：5

共 25 条

[1] ANTISENSE DNA OLIGONUCLEOTIDES .2. THE USE OF MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY FOR THE SEQUENCE VERIFICATION OF METHYLPHOSPHONATE OLIGODEOXYRIBONUCLEOTIDES
KEOUGH, T
BAKER, TR
DOBSON, RLM
LACEY, MP
RILEY, TA
HASSELFIELD, JA
HESSELBERTH, PE
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (03) : 195 - 200
[2] MASS AND SEQUENCE VERIFICATION OF MODIFIED OLIGONUCLEOTIDES USING ELECTROSPRAY TANDEM MASS-SPECTROMETRY
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VOUROS, P
VANSCHEPDAEL, A
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JOURNAL OF MASS SPECTROMETRY, 1995, 30 (07): : 993 - 1006
[3] SEQUENCE-ANALYSIS OF OLIGODEOXYRIBONUCLEOTIDES BY MASS-SPECTROMETRY .1. DINUCLEOSIDE MONOPHOSPHATES
WIEBERS, JL
SHAPIRO, JA
BIOCHEMISTRY, 1977, 16 (06) : 1044 - 1050
[4] Mass and sequence verification of modified oligonucleotides using electrospray tandem mass spectrometry
J Mass Spectr, 7 (993):
[5] USE OF MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY FOR SEQUENCING ANTISENSE DNA OLIGONUCLEOTIDES
KEOUGH, T
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ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 208 : 160 - ANYL
[6] Electrospray tandem mass spectrometry of mixed-sequence RNA/DNA oligonucleotides
Dept. of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
不详
J. Am. Soc. Mass Spectrom., 1600, 8 (936-945):
[7] Electrospray tandem mass spectrometry of mixed-sequence RNA/DNA oligonucleotides
Schürch, S
Bernal-Méndez, E
Leumann, CJ
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2002, 13 (08) : 936 - 945
[8] TANDEM MASS-SPECTROMETRY OF PEPTIDES - SEQUENCE INFORMATION-BASED ON NEUTRAL LOSSES .1. ISOMERIC DIPEPTIDES
CORDERO, MM
WESDEMIOTIS, C
ORGANIC MASS SPECTROMETRY, 1993, 28 (10): : 1041 - 1046
[9] ANALYSIS OF 5-METHYLCYTOSINE IN DNA .1. MASS-SPECTROMETRY
DEUTSCH, J
RAZIN, A
SEDAT, J
ANALYTICAL BIOCHEMISTRY, 1976, 72 (1-2) : 586 - 592
[10] SEQUENCE VERIFICATION OF HUMAN CREATINE-KINASE (43 KDA) ISOZYMES BY HIGH-RESOLUTION TANDEM MASS-SPECTROMETRY
WOOD, TD
CHEN, LH
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BABBITT, PC
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (25) : 11451 - 11455

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